Purification and properties of a glycerol ester hydrolase (lipase) from Propionibacterium shermanii.

Carnitine ester hydrolase of rat liver.

Rat liver, kidney, pancreas, and small intestine have enzymes which hydrolyze fatty acid esters of carnitine. The liver enzyme, located in the microsomal fraction, was solubilized by sonic disruption and purified l&fold by diethylaminoethyl cellulose chromatography and Sephadex gel filtration. The purified enzyme sedimented in the ultracentrifuge as a single protein with an szo+, of 4.5 S. The ...

Propionic acid production in glycerol/glucose co-fermentation by Propionibacterium freudenreichii subsp. shermanii.

Propionibacterium freudenreichii subsp. shermanii can ferment glucose and glycerol to propionic acid with acetic and succinic acids as two by-products. Propionic acid production from glucose was relatively fast (0.19 g/Lh) but gave low product yield (~0.39 g/g) and selectivity (P/A: ~2.6; P/S: ~4.8). In contrast, glycerol with a more reduced state gave a high propionic acid yield (~0.65 g/g) an...

Lipase of Mucor pusillus.

Lipase of Mucor pusillus NRRL 2543 was recovered with ammonium sulfate precipitation, gel filtration on Sephadex G-75, and anion-exchange chromatography on diethylaminoethyl-Sephadex A-50. Maximal glycerol ester hydrolase (lipase) activity was observed at pH 5.0 to 5.5 and 50 C when trioctanoin and olive oil were used as substrates. The enzyme also showed esterase activity; it hydrolyzed, with ...

بررسی تاثیر متابولیت های Propionibacterium shermanii بر ویژگی های حسی و ماندگاری پنیر فتای UF

Purification and properties of a phosphorylatable triacylglycerol lipase from the fat body of an insect, Manduca sexta.

A triacylglycerol lipase, presumably the first enzyme involved in the mobilization of lipid from the insect fat body, has been purified to homogeneity from the fat body of Manduca sexta. The purification procedure involved polyethyleneglycol precipitation, and chromatography on DEAE-cellulose, phenyl-Sepharose, Q-Sepharose and hydroxylapatite. The final product, a protein with an M(r) = 76,000 ...